高原型藏绵羊附睾细胞外基质的组织化学研究

doi:10.11843/j.issn.0366-6964.2016.04.023

摘要/Abstract

摘要：

旨在探索高原型藏绵羊附睾细胞外基质相关蛋白的分布特征。应用Masson’s、Gomori’s、PAS和AB-PAS（pH 2.5）染色组织化学方法观察7只健康成年高原型藏绵羊附睾的组织结构特点，进而用免疫组织化学SP法观察层粘连蛋白（LN）、IV型胶原蛋白（Col IV）和硫酸乙酰肝素蛋白多糖（HSPG）的分布特征。高原型成年藏绵羊附睾各部分管腔为柱状纤毛上皮，附睾尾间质胶原纤维和网状纤维较附睾头及附睾体明显增多。PAS反应显示附睾尾阳性强于附睾头和附睾体，AB-PAS反应显示附睾头和附睾尾的阳性强于附睾体。免疫组织化学结果显示，LN在组织中表达最强，HSPG次之，Col IV表达较弱，HSPG和LN的阳性表达差异不显著（P>0.05）。高原型成年藏绵羊附睾尾间质胶原纤维和网状纤维的分布较附睾头和附睾体多，附睾各部分中PAS的反应强弱与附睾上皮分泌功能的变化密切相关，附睾尾输精管的分泌功能增强；Col IV参与基膜的物质转运；HSPG与血-附睾屏障构成相关；LN与基膜的形成及其附睾中细胞外基质相关蛋白（ECM）的合成和分泌有关。

Abstract:

To explore the distribution of extracellular matrix proteins of epididymis in Plateau Tibetan sheep.The histochemistry of Masson’s，Gomori’s，PAS and AB-PAS（pH 2.5）stainning were used to study the microstructure of epididymis in 7 Plateau Tibetan sheep，also the distribution of the laminin (LN)，type IV collagen (Col IV) and heparan sulfate glycoprotein (HSPG) was identified by SP immunohistochemical method.The observations made with the light microscope showed that the epithelium of epididymal ducts were columnar ciliated epithelium and the cauda was more abundant with collagen and reticular fiber than in the caput and the corpus，and as well as the PAS positive reaction in the cauda，but AB-PAS positive reaction showed that the caput and the cauda were stronger than the corpus.In addition，immunostaining analysis appeared that LN was strongly presented in the epididymis，the relative expression of HSPG was lower than LN，and Col IV was weakly expressed，but the distribution of LN and HSPG without statistical differences in epididymis (P＞0.05).Taken together，the collagen and reticular fiber were more abundant in cauda than caput and corpus in Plateau Tibetan sheep epididymis，and the intensity of the PAS reaction was companied with the changes of the secretion function of epithelium，the Col IV distribution of ECM proteins was participated in basement membrane transport，but HSPG was related to the blood-epididymis barrier，and the distribution of LN may closely related with the membrane construction and secretion the of other extracellular matrix proteins in epididymal ducts.

中图分类号:

李聪，袁莉刚，曲亚玲，鲁玉荣，路琪中. 高原型藏绵羊附睾细胞外基质的组织化学研究[J]. 畜牧兽医学报, 2016, 47(4): 820-827.

LI Cong，YUAN Li-gang，QU Ya-ling，LU Yu-rong，LU Qi-zhong . The Histochemical Study of Extracellular Matrix in Plateau Tibetan Sheep Epididymis[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(4): 820-827.

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